Selection of Astaxanthin-Overproducing Mutants of Phaffia rhodozyma with beta-Ionone.
نویسندگان
چکیده
beta-Ionone, an end ring analog of beta-carotene, inhibits astaxanthin production in the red yeast Phaffia rhodozyma. Astaxanthin-overproducing mutants of this yeast are easily spotted on beta-ionone-containing yeast malt agar plates. beta-Ionone appears to block astaxanthin synthesis at the beta-carotene level.
منابع مشابه
Overexpression of a bifunctional enzyme, CrtS, enhances astaxanthin synthesis through two pathways in Phaffia rhodozyma
BACKGROUND A moderate-temperature, astaxanthin-overproducing mutant strain (termed MK19) of Phaffia rhodozyma was generated in our laboratory. The intracellular astaxanthin content of MK19 was 17-fold higher than that of wild-type. The TLC profile of MK19 showed a band for an unknown carotenoid pigment between those of β-carotene and astaxanthin. In the present study, we attempted to identify t...
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Fed-batch essays using yeast Phaffia rhodozyma were performed to obtain high astaxanthin concentrations. The yeast was grown over an inexpensive media obtained from the Yucca fillifera date. Feeding was carried out through implantation of a pH-stat control system. Biomass (28.7 mg/l) and pigment (18 mg/l) concentration three folded those that were obtained by previous batch cultures.
متن کاملThe effect of gamma irradiation on astaxanthin synthetase encoding gene in two mutant strains of Phaffia rhodozyma
BACKGROUND AND OBJECTIVES Astaxanthin, an orange-red carotenoid pigment, acts as a protective agent against oxidative damage to cells in vivo. The astaxanthin synthetase gene (crtS) size consists of 3995 bp. This gene has been suggested to catalyse β-carotene to astaxanthin in Phaffia rhodozyma. The aim of this research was to find any possible changes in this gene in two mutant strains, Gam1 a...
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BACKGROUND AND OBJECTIVES Phaffia rhodozyma is a red yeast which produces astaxanthin as the major carotenoid pigment. Astaxanthin is thought to reduce the incidence of cancer and degenerative diseases in man. It also enhances the immune response and acts as a free-radical quencher, a precursor of vitamin A, or a pigment involved in the visual attraction of animals as mating partners. The impac...
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BACKGROUND Growth conditions that bring about stress on Phaffia rhodozyma cells encourage the synthesis of astaxanthin, an antioxidant carotenoid, which protects cells against oxidative damage. Using P. rhodozyma cultures performed with and without copper limitation, we examined the kinetics of astaxanthin synthesis along with the expression of asy, the key astaxanthin synthesis gene, as well a...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 56 9 شماره
صفحات -
تاریخ انتشار 1990